Unigreen Master UMP 20 Guida Utente

305 PumpUser’s Guide

1-2Introduction 1Using this ManualUsing this ManualThe 305 piston pump is a precision instrumentwhich is simple and easy to use. To gain themaximum fr

Appendix E-4305 Programming Sheet Appendix E

Appendix F-1Technical Data Appendix FThe following information presents construction and operational characteristics forGilson 305 Pumps.

Appendix F-2Technical Data Appendix FType of PumpType of PumpProgrammable reciprocating pump with single-piston interchangeable head, constant stroke,

Appendix F-3Technical Data Appendix FWorking Range & Performance DataWorking Range & Performance DataWorking Range, Pump Heads andassociated M

Appendix F-4Technical Data Appendix FWorking Range & Performance DataLiquid-contact Materials316L stainless steel, titanium, sapphire/ceramic,ruby

Appendix F-5Technical Data Appendix FControl and InterfacesControl and InterfacesOperation ModesConstant flow rate (Flow), constant volume(Dispense),

Appendix F-6Technical Data Appendix FControl and InterfacesElectrical Interface4 inputs, 3 programmable outputs.Digital InterfaceGilson Serial Input/O

Appendix F-7Technical Data Appendix FEnvironmental ConditionsEnvironmental ConditionsStorageIndoor use only.Installation : Category II.Altitude: Up to

Appendix F-8Technical Data Appendix F

1-3Introduction 1UnpackingUnpackingThe 305 piston pump is packed in a single carton.Upon receipt of your instrument, carefully unpackthe unit and insp

World Wide Web: www.gilson.comE-mail: [email protected], [email protected], [email protected] HeadquartersGilson, Inc.3000 W. Beltline Hwy., P.

1-4Introduction 1

2-1Description 2This chapter describes the physical layout of the 305 piston pump. It describes the mainbody of the 305 and the position of the electr

2-2Description 2Front ViewFront viewThe figure below shows a front view of the 305 Masterpump with a pump head mounted. There is a keypadwhich consist

2-3Description 2KeypadKeypadThe figure below shows the keypad with thenumeric keys, the display and the soft keys.Power-on indicator DisplaySoftkeysPR

2-4Description 2Rear ViewThe figure below shows a rear view of the 305 withthe electrical connectors. The function of eachconnector is as follows:• GS

3-1Installation 3This chapter describes how to install the 305 Master pump. It is recommended that youfollow the installation instructions in the orde

3-2Installation 3Electrical Installation - PowerElectrical Installation - PowerFor safety reasons, the 305 is shipped without thefuses installed and w

3-3Installation 3Electrical Installation - PowerTo set the voltage to 220/240 volts:Insert the fuse holder with the numbers 220/240 onthe bottom, faci

3-4Installation 3Mechanical InstallationThis section explains how to install the pump head,the mast clamp and mast. The pump head and mastclamp for ea

3-5Installation 3Mechanical InstallationFix one clamp onto each pump in the system. Afterall the modules have been positioned, the stainlesssteel mast

3-6Installation 3Positioning the ModulesPositioning the ModulesBefore putting each module in position, make surethat each module is ready, i.e. that t

3-7Installation 3Positioning the ModulesAuto-Preparative SystemThis is a binary gradient system with two elutionpumps, one injection pump, one manomet

3-8Installation 3Control ConnectionsControl ConnectionsAfter positioning all of the modules in the system, it isnecessary to connect each module to th

3-9Installation 3looking at the rear of the 306 pumps and ensuringthat the correct switch (2, 3 or 4) has been switchedinto the bottom position. The s

3-10Installation 3Hydraulic ConnectionsHydraulic ConnectionsThe hydraulic connections for the 305 pump headare made using the tubing provided in the s

3-11Installation 3Input/Output ConnectionsInput/Output ConnectionsFor coordination with surrounding equipment,electrical contacts are used.The input/O

3-12Installation 3The Pause/Resume InputThe Pause/Resume input is used topause and restart the 305 pump usingan external contact.This input is only ac

3-13Installation 3The IN # 2 InputThis input is activated when closed. Activatingthis input while Program mode is selected, causesFile 13 to run. If n

305 Piston PumpUser’s GuideLT801152M/©2003 Gilson SAS All rights reserved September 2003

3-14Installation 3

4-1Operation 4After switching ON the 305, the display shows thefollowing information for one second:This indicates the pump version, software version,

4-2Operation 4Priming the Pump HeadPriming the Pump HeadDo not run the pump when the pump head is dry.This can result in severe pump head damage.Check

4-3Operation 4Using the KeypadUsing the KeypadThe keypad consists of numeric keys, dedicatedkeys such as Enter and Prime, 5 white soft keys anda 2 lin

4-4Operation 4Setting Up the PumpThe software for the 305 Master pump can beexplained with the help of the chart in Figure 15.The chart has 4 software

4-5Operation 4With a new pumping system, you must enter dataabout each pump connected, i.e. Refill Time, solventCompressibility and pump Head Size and

4-6Operation 4Pump A Refill timeThe refill time is the time required for the pistonreturn stroke. Normally it is set at the lowest value(125 ms) to gi

4-7Operation 4Setting Up the PumpPump A CompressibilityThis data is used to calculate the flow rate compen-sation for the compressibility of the solve

4-8Operation 4A table of inlet pressure values for CO2 is shownbelow.• Key in the desired inlet pressure according to theambient temperature.• Press E

4-9Operation 4Input/Output Parameter Set Up (I/O)The I/O menu is used to enter data about param-eters associated with the complete system. To go tothe

4-10Operation 4Setting Up the Pump# The default value is 0.• Key in the value that applies to your system andpress Enter.This menu will not appear ift

4-11Operation 4AlarmThe alarm is a buzzer which sounds every timethere is an error or an invalid setting entered orencountered. It can be programmed t

4-12Operation 4By using this procedure, each of the three outputscan be manually set to be open or closed. Theoutputs will remain in this state until

4-13Operation 4Delay VolumeWhen the selected gradient profile is a solventcomposition (%B for instance), the Delay Volumecan be used to synchronise on

4-14Operation 4• Set the GSIOC ID number for the 305 Master pump.• Program outputs to be Open/Closed.• Set pause in program to be with or without flow

4-15Operation 4Mode SelectionMode SelectionRunning the Pump in Flow ModeIn Flow mode, the pump provides a constant flowrate, commencing when the Run s

4-16Operation 4• The pump will start either when the Run soft keyis pressed, or when the start input is activated.• The pump will stop when the Stop s

4-17Operation 4Mode SelectionRunning the Pump in Dispense ModeIn this mode the pump can be used to deliver aspecified volume beginning when the Run so

4-18Operation 4The maximum dispense flow rate depends on theRefill Time and Compressibility. If the parametersare not compatible with the dispense flo

4-19Operation 4Operation of the Pressure LimitsAs a safety feature of the system, it is possible todefine high pressure and low pressure limits so tha

Table of Contents-1Table of ContentsSafety1 IntroductionUsing this Manual ... 1-2

4-20Operation 4If the pressure falls below the low pressure limit,the pump will stop and the message Low pressurelimit will flash on the screen. The a

4-21Operation 4At the end of a run in Program mode, the SolventConsumption can be obtained by selecting theappropriate roll screen.The 305 controls up

4-22Operation 4File SelectionOne complete method is stored in a file. In order toread/edit/write a method, you have to go to a file.The sequence of sc

4-23Operation 4DirectoryPress this key to go to each stored file, e.g. File 1,File 3 etc. This displays all of the files where methodprograms are stor

4-24Operation 4Programming a MethodA complete method program is written by pro-gramming flow rates, solvent compositions andoperation of the outputs.

4-25Operation 4Programming a MethodIf you link files together which have differentvalues for the setup parameters, the pump will notstart. After press

4-26Operation 4The percentage of solvent A is calculated as:% solvent A = 100 - % B - %C.The example opposite is for a binary gradient.At 0 minutes, t

4-27Operation 4• key in the value for the flow rate, 0, and pressEnter.• press the Add soft key to add a second timedevent.• key in the time for the s

4-28Operation 4- Time at which the pump will wait for an input- Waiting for an open contact or a closed contactExample 4: 2.00 min Wait #1 Closed.To p

4-29Operation 4Example: Screen 1 3.00 min Output 2Screen 2 Open Close PulseTo program this example:• press the Add soft key.• press the Out soft key.

Table of Contents-2File Selection ... 4-22Directory...

4-30Operation 4At the selected time, the Master 305 starts theinjection pump and automatically lowers theelution rate in order to keep the total flow

4-31Operation 4Programming the Safety FilesThe Safety Files are a useful safety feature whichcan be used to control the pumping system if a mal-funct

4-32Operation 4High Pressure Safety File (File 12)If the pressure goes above the high pressure limit,the sequence of events is as follows:• If the hig

4-33Operation 4If there is more than one file in the method, i.e.one file is linked to another, the program will goback to the beginning of the first

4-34Operation 4Running a Method ProgramAt the end of programming the method and errorfiles, the software returns to a Ready-to-RunScreen. The top left

4-35Operation 4Press Run to start the conditioning of the column.The pump will ramp to the flow rate and solventcomposition that is programmed in the

4-36Operation 4When the file is being run again, if any of theseparameters has been changed, the following mes-sage will appear after Run has been pre

4-37Operation 4During a method run, the setup parameters andthe method program can be modified. Modify aparameter in the same way that you program i

4-38Operation 4Programming ExamplesProgramming ExamplesTwo example programs are given below. Beforerunning these example programs, ensure that thehydr

4-39Operation 4Simple programs are given in list two and three forthe Low pressure error file and the High pressureerror file. In both programs, the f

Safety-1SafetyRead this section carefully before installing and operating the pump.For safe and correct use of the pump, it is essential that both ope

4-40Operation 4Example 2The operation of the method in Example 2will be as follows.File # 4 contains the method program. Theflow rate remains constant

4-41Operation 4To improve on this basic program, add a wait forinput # 1 timed event at 0.00 min. This means thatthe gradient will not start until it

4-42Operation 4

5-1Maintenance and Troubleshooting 5The 305 pump has been designed to require a minimum level of care andmaintenance. In practice, maintenance is limi

5-2Maintenance and Troubleshooting 5Pump Head MaintenancePump Head MaintenanceThe check valves and filters can be cleaned. Pistonseals, check valves,

5-3Maintenance and Troubleshooting 5TroubleshootingTroubleshootingElectrical ProblemsProblem Possible cause SolutionPump does not operate and Power co

5-4Maintenance and Troubleshooting 5TroubleshootingHydraulic problemsProblem Possible cause SolutionLeaks from the hole at theDefective piston seal. R

Appendix A-1Accessory Parts List Appendix AParts lists for the 305 Pump, consisting of Standard Accessories and AdditionalAccessories.

Appendix A-2Accessory Parts List Appendix AStandard Accessory Parts ListStandard Accessory Parts ListReference Qty Description3645388 1 SC type pump h

Appendix A-3Accessory Parts List Appendix AAdditional Accessory Parts ListAdditional Accessory Parts ListReference Description36078143 GSIOC cable7099

Safety-2SafetyHowever, adequate protection including clothingand ventilation must be provided if dangerousliquids are used. In case of incidental spil

Appendix A-4Accessory Parts List Appendix A

Appendix B-1GSIOC Control Appendix BThis chapter explains how to control the 305 from a computer using Gilson HPLCsystem controller software.

Appendix B-2GSIOC Control Appendix BGSIOC FeaturesGSIOC FeaturesGSIOC stands for Gilson Serial Input OutputChannel. This communications channel links

Appendix B-3GSIOC Control Appendix BGSIOC CommandsGSIOC CommandsThe GSIOC commands can be used to controlGilson modules directly from a computer or fr

Appendix B-4GSIOC Control Appendix BImmediate I Read Contact InputsResponse format: “abcd” wherea is the START/STOP input,b is the PAUSE input,c is th

Appendix B-5GSIOC Control Appendix BImmediate j Read Contact Output BuffersResponse format: “abcde” wherea is the OUT #1 buffer,b is the OUT #2 buffer

Appendix B-6GSIOC Control Appendix BBuffered Q Enter Pressure ValueSyntax: “QBxx.x” in bars, “QPx.xx” in MPa, or “QKxx.x” in kpsi.Parameter: if xxx is

Appendix B-7GSIOC Control Appendix BGSIOC CommandsIdentification of the master pump (pump A) is notcritical. However, if a Model 305 is used with acom

Appendix B-8GSIOC Control Appendix BGSIOC CommandsExample 4: to restore display messagesThis sequence sends “reconnect”commands to the internal softwa

Appendix C-1Twin-pump Systems Appendix CIn each of the systems outlined in this manual there is a maximum of four pumps. Thesignals from the controlle

1-1Introduction 1Gilson’s 305 Master pump conform to the standard specified in the ‘Declaration ofConformity’ certificate (reference LT801354) supplie

Appendix C-2Twin-pump Systems Appendix CTwo Possible Cases:- A system of mirror pumps controlled by a 305Master pump.- A system of mirror pumps contro

Appendix D-1Reference InformationsAppendix DThis chapter contains Table of Solvent Miscibility, Liquid Compressibility Values andFlow Rate Accuracy Pr

Appendix D-2Reference Informations Appendix DSolvent Miscibility TableMiscibility means that solvents should mix with eachother in all proportions. Th

Appendix D-3Reference Informations Appendix DLiquid Compressibility ValuesBibliography DataThe values of isothermalcompressibility given belowcan be u

Appendix D-4Reference Informations Appendix DLiquid Compressibility ValuesOther DataFor other liquids currently used atambient temperatures (20-25 °C)

Appendix D-5Reference Informations Appendix DFlow Rate Accuracy PrincipleFlow Rate Accuracy PrincipleTo generate the selected flow rate with high accu

Appendix D-6Reference Informations Appendix DThe complementary compensation flow rate for allother factors, F2, is defined as the difference:F2 = F (F

Appendix E-1305 Programming SheetAppendix EThe programming sheets provided on the next pages should be properly filled outprior to programming the pum

Appendix E-2305 Programming Sheet Appendix ESet Up ParametersStep No Time Event Operation Step No Time Event Operation11321431541651761871982092110 22

Appendix E-3305 Programming Sheet Appendix EExample